The invention discloses a culturing method for orchid. Through hardening tissue culture vessel seedlings in a seedling-hardening substrate, and middle and big seedlings are obtained through one or more years of culturing and then planted into a culture medium to be cultured, so that adult seedlings of the orchid are obtained. The culturing method and the substrate have the advantages of good water retention, fertilizer maintenance and moisture preservation; cleanness is achieved in the using process, harmful bacteria are not prone to being generated, good growth of the seedlings is guaranteed, and the growth of the orchid is facilitated; the substrate is made of agricultural waste, is ecological and environmentally friendly and saves the cost. The substrate increases the survival rate of hardened tissue culture seedlings of the orchid, the transplanting survival rate reaches 95% or above, growth speed is high, and the seedlings start to take root and come into leaves about 30 days after transplanting. The growth of the middle and big seedlings is promoted, the uniformity of the seedlings is improved, blooming is advanced, potting quality is excellent, and the production cost is lowered. The culturing method has the advantages that the production period is short, the production cost is low, air permeability and water permeability are good, and collecting and transporting are facilitated; the agricultural waste is effectively utilized for reducing deforestation, and therefore the environment is protected.