The invention discloses traditional Chinese medicine composition with efficacy of inhibiting generation of lactic acid and a preparation method of the traditional Chinese medicine composition. The composition is prepared from raw materials including hedyotis hedyotidea, sarmentose pepper herbs, smilax riparia, twotooth achyranthes roots, Parabarium chunianum, leaves and twigs of Fruticose Breynia, leaves of Chinese desmos, herba of Inula cappa DC., diverse wormwood herbs, Entada phaseoloides stems, Hyptis suaveolens, radix cudraniae, zanthoxylum nitidum var. tomentosum, suberect spatholobus stems, twigs and leaves of common claoxylon, roots of solanum surattense Burm. f., pubescent angelica roots, semen impatientis, fruits of largeflower gardenia, cloves, nutgrass galingale rhizomes, roots of Kadsura coccinea, herba centipedae, lindera aggregate, Lobelia dauidii Franch., prepared kusnezoff monkshood roots, camphor, herba hyperici japonici, roughhaired holly roots, cochinchina momordica seeds, rhizoma atractylodis and cassia twigs. The invention further provides a preparation method of the traditional Chinese medicine composition with the efficacy of inhibiting generation of lactic acid.