The invention belongs to the technical field of function materials, and discloses a production method of a sandwich solid phase extraction membrane. The method comprises the following steps: filtering adsorbent particles by a base film to form a nanometer or micrometer thickness adsorbent layer, carrying out knife coating on the surface of the adsorbent layer with a membrane solution, and carrying out immersion deposition or a sol-gel process to obtain a porous surface membrane in order to obtain the sandwich solid phase extraction membrane. The base membrane has a supporting effect, the surface membrane has adsorbent layer fixing and protecting and liquid distributing effects, and the obtained solid phase extraction membrane has high adsorption capacity and large integral strength, can be used in enrichment of a trace quantity of substances in a liquid and adsorption and separation of a trace amount of substances, and can be used to sample analysis pretreatment and adsorption treatment of pollutants in water.