The present invention discloses a ketal sugar alcohol-based micromolecular gelator and preparation method and application thereof. The method is as below: reacting a hexahydric sugar alcohol matrix with 2,2-dimethoxypropane, and modifying lipophilic groups to achieve lipophilic modification on the gelator, thereby preparing the ketal sugar alcohol-based micromolecular gelator. The ketal sugar alcohol-based micromolecular gelator prepared by the invention can optionally gelatinize an oil phase in a water-oil mixed system, conduct self-assemble by intermolecular hydrogen bond and other non-covalent interactions to form a physical crosslinking supramolecular structure, and fix the oil phase by surface tension and capillary forces, so as to reach the gelatinization effect. The simple preparation process does not need heating or addition of additives in the application process, and is fast, efficient and environment-friendly.