The invention discloses a preparing, regulating and control method for a stable nanometer pattern based on a cross-linkable high polymer material. A segmented copolymer with an active cross-linkable nature is used for forming a nanometer pattern of a precise structure through self-assembly firstly, and then cross-linkable functional groups of the segmented copolymer are cross-linked through measures such as ultraviolet light irradiation, the stable and precisely-arranged nanometer pattern is obtained, and the precise nanometer pattern is immobilized. The method is low in preparing cost, simple in process, precise, controllable and easy to apply and popularize, and has very wide application prospects in multiple fields such as medical science, microelectronics, chemistry and material science.