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发明公开
CN105648119A 检测猪繁殖与呼吸障碍综合症病毒的引物、试剂盒及方法 有权

检测猪繁殖与呼吸障碍综合症病毒的引物、试剂盒及方法
摘要：
本发明公开了一种检测猪繁殖与呼吸障碍综合症病毒的引物、试剂盒及方法。所述引物如SEQ ID NO:1和SEQ ID NO:2所示，所述方法为以待检测样品的RNA为模板，进行RT-PCR扩增反应，电泳鉴定。本发明的检测猪呼吸与繁殖障碍综合症病毒的引物具有很高的特异性和灵敏度，能够检测出猪繁殖与呼吸障碍综合症的新型病毒类NADC30病毒；PCR扩增可以通过扩增片段的大小不同快捷的对PRRSV的经典株、变异株和类NADC30毒株这3类毒株进行鉴别区分，省去测序的步骤，节省了时间和成本，操作简单、实用。
摘要（英）：

The invention discloses a primer, kit and method for detecting porcine reproductive and respiratory syndrome viruses. The primer is as shown in SEQ ID No.1 and SEQ ID No.2. The method includes: using the RNA of a to-be-detected sample as the template, performing RT-PCR amplification reaction, and performing electrophoresis identification. The primer, kit and method has the advantages that the primer is high in specificity and sensitivity and capable of detecting the novel NADC30 viruses of the porcine reproductive and respiratory syndrome; PCR amplification can fast identify the classical strains, variants and strains similar to NADC30 of the PRRSV (porcine reproductive and respiratory syndrome viruses) through the size differences of amplification fragments, the sequencing step is omitted, time and cost are saved, and simpleness in operation and practicality are achieved.

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