The invention relates to a real-time analysis method and system of flash memory interface information. An information acquisition condition and a trigger point are set, the flash memory interface information is obtained, the flash memory interface information is filtered according to the information acquisition condition and the trigger point, and the required flash memory interface information is acquired; according to the communication protocol of a flash memory interface, carrying out real-time identification analysis on the collected flash memory interface information to identity and analyze specific meanings expressed by each-bit data, wherein the specific meanings comprise the type of a control signal and one or multiple of expressed meanings and data lengths. The real-time analysis method and system of flash memory interface information can realize real-time processing. In addition, data can be properly processed on a data acquisition end so as to greatly improve data analysis efficiency, and flexible configuration can be carried out according to the demands of a user.