The invention relates to an ultrasonic contrast agent of a uterus oviduct tract and a preparation method of the ultrasonic contrast agent. The ultrasonic contrast agent is prepared from the following components: a first component is a carrier and is composed of phospholipid, polyethylene glycol or a derivative thereof and polymerized phospholipid connected with a specific ligand at the weight ratio of (0.5 to 2):(0.005 to 0.02):(0.003 to 0.007); a second component is a phospholipid stabilizer; a third component is a biological solvent; a fourth component is a fluorocarbon gas; the weight percent ratio of the first component to the second component to the third component is (0.0005 to 0.002):(0.000005 to 0.00002):(0.5 to 2); the concentration of the fourth component which is gas is 10<8>-10<9>/mL.