The invention relates to a method for industrially preparing chromatographic separation and purification of Z6148 liquid crystal molecules, and belongs to the technical field of liquid crystal materials. The method comprises the following steps that 1, the Z6148 liquid crystal molecules are synthesized a reaction solution for preliminary purification; 2, the preliminarily-purified Z6148 liquid crystals are dissolved into a solvent to form a solution, and filtering is performed to remove insoluble substances; 3, the Z6148 liquid crystal solution is pumped into a dynamic axial compression column to prepare a chromatographic system, mobile phase gradient elution is performed, and an ultraviolet luminosity detector with the detection wavelength being 254 nm is used for collecting a distillate with the retention time being 30-50 min. The adopted solvent is low in cost and safe, the separation and purification process is completed in one step, online real-time monitoring can be performed, safety is greatly improved, raw materials and time cost are saved, and the method is applicable to large-scale industrial production.