The invention relates to an anti-reverse device for a comber cylinder shaft. The anti-reverse device comprises a stop seat, the cylinder shaft, a non-return device and a comber wallboard, wherein the non-return device used for preventing reverse rotation of the cylinder shaft is arranged on the cylinder shaft and mounted in a hole of the stop seat; and the stop seat is fixed to the comber wallboard of the anti-reverse device. According to the anti-reverse device, the non-return device arranged on the cylinder shaft ensures that the cylinder shaft does not rotate reversely when a machine stops due to a fault in a working process.