The invention relates to a titanium dioxide (TiO2) fiber paper for cryogenic insulation and a manufacturing method and application thereof.The titanium dioxide fiber paper comprises TiO2 which is not less than 90% by mass percentage and diameter of TiO2 fibers ranges from 0.3 micrometer to 2.5 micrometers.The manufacturing method includes the steps of (1), cutting the TiO2 fibers into short fibers, dispersing the short fibers in pure water or/and clean water, adding in binder with stirring and dispersing uniformly to obtain a slurry; (2), removing residue of the slurry and then spreading the slurry on a net by a beater, dewatering by suction filtration, pressing, and drying to obtain the product.The titanium dioxide fiber paper with high radiation capacity and high heat insulation can be composited with zirconia fibers, has low outgassing rate in vacuum, and has stable property at low temperature when used for coating containers of liquid nitrogen, liquefied natural gas and the like.