A construction process of a coiled tubing operation sledge assembly comprises the steps that 1 installation of the operation sledge assembly, 2 injection of a coiled tubing, 3 fixing of the coiled tubing and 4 moving out of the operation sledge assembly. By the adoption of the construction process of the coiled tubing operation sledge assembly, coiled tubing removal can be performed, meanwhile the operation sledge assembly can be moved to other well mouths for operation under the situation that the coiled tubing is not cut off, operation flexibility is good, the device utilization rate is high, the operation reliability is good, the demands of an operation site are further met, the device using amount is small, the operation cost is low, the coiled tubing is not needed to be damaged, the consumption of the coiled tubing is reduced, and the cost is further reduced.