The invention discloses a method for detecting dopamine by using carbon dots as a fluorescence probe. The method comprises the following steps: preparation of a fluorescence probe, establishment of a standard curve and determination of dopamine concentration. Carbon dots with high luminescent efficiency are used as the fluorescence probe, and trace amount of dopamine is efficiently detected by the fluorescence enhancement of dopamine on the carbon dots. The detection limit reaches 0.1 pM, detection sensitivity is high, and selectivity is good.