The invention discloses a temperature control device, comprising a pallet and a rotating shaft driving the pallet to rotate; the top end face of the pallet is provided with a plurality of reagent bits and a plurality of sample bits, and the bit reagent bits and sample bits are all blind holes; a first heating resistance wire is arranged in the gap between the rotating shaft and the reagent bits, and is used for heating reagents in the reagent bits; the periphery of the sample bits is provided with a second heating resistance wire, and the outside of the second heating resistance wire is provided with an insulation protection layer; the second heating resistance wire is used for heating samples in the sample bits; the bottom of the pallet is a hollow structure, and comprises a first chassis and a second chassis; a thermal cooler is arranged between the first chassis and the second chassis, and clings to the bottom of the first chassis. The temperature control device has the characteristics of high temperature adjusting efficiency and temperature control precision, and low cost.