Provided is a touch screen. The touch screen comprises base material, an anti-reflective film and a conductive layer, all of which are overlapped in order. The anti-reflective film comprises at least one layer with high refractive index and at least one layer of low refractive index, all of which are laminated in an alternating mode. Due to the structure of the anti-reflective film between the base material and the conductive layer, OCA/ITO and OCA/PET interfaces are avoided so that reflection is effectively reduced. The anti-reflective film comprises at least one layer with high refractive index and at least one layer of low refractive index, all of which are laminated in an alternating mode. Therefore, light transmission of the conductive layer is improved so that reflection of a visible region of the touch screen is further and effectively reduced. The color difference between the visible region and the non-visible region is eliminated so that display quality is improved. At the same time, the invention provides a manufacture method of the touch screen.