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发明公开
CN105683375A 用于插入核酸的载体 有权

用于插入核酸的载体
摘要：
本发明提供：一种载体，其用于在从5’末端到3’末端的方向依次包含由第1核苷酸序列构成的区、规定部位和由第2核苷酸序列构成的区的核酸的上述规定部位插入所期望的核酸，该载体在从5’末端到3’末端的方向依次包含由第1核苷酸序列构成的区、上述所期望的核酸和由第2核苷酸序列构成的区；包含该载体的试剂盒；核酸的插入方法，该方法包括向细胞中导入该载体的步骤；通过该方法获得的细胞；以及包含该细胞的生物。
摘要（英）：

The present invention provides the following: a vector for inserting a desired nucleic acid into a predetermined site of a nucleic acid comprising a region formed of a first nucleotide sequence, the predetermined site, and a region composed of a second nucleotide sequence, in the stated order in the 5'-to-3' direction, wherein the vector comprises a region formed of the first nucleotide sequence, the desired nucleic acid, and the second nucleotide sequence in the stated order in the 5'-to-3' direction; a kit that includes this vector; a method of inserting a nucleic acid comprising a step for introducing this vector into a cell; a cell acquired by this method; and an organism comprising this cell.

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