The invention discloses a preparation method and a special culture solution for artificial seeds of broussonetia papyifera. The invention firstly provides the preparation method for the artificial seeds of the broussonetia papyifera. The preparation method comprises the following steps of: (a1) incubating somatic embryos of the broussonetia papyifera in a culture solution IV, wherein the culture solution IV is prepared by adding 1.8-2.2mg of 6-BA, 0.9-1.1mg of NAA, 18-22g of saccharose, 0.9-1.1g of lower-molecular weight hitosan, 0.9-1.1g of oxalic acid and 36-44g of sodium alginate into per litre of 1/10MS culture medium; and (a2) adding a system obtained in the step (a1) into a 0.9-1.1g/100mL CaCl2 aqueous solution, and incubating to obtain the artificial seeds of the broussonetia papyifera. The invention further provides a culture solution for preparing the artificial seeds of the broussonetia papyifera, wherein the culture solution is the culture solution IV. The preparation method and the special culture solution are capable of realizing efficient propagation of broussonetia papyifera seedlings and meeting the market requirement of the broussonetia papyifera seedlings, and have great promotional value.