The invention discloses an industrialized seedling culture method for detoxified health sugarcane seedlings. According to the industrialized seedling culture method, a sealing cover with ventilating and bacteria filtering functions is utilized for replacing conventional closed covers to seal a culture container in each indoor seedling culture stage of the detoxified health sugarcane seedlings; and by virtue of the ventilating sealing cover, the culture container has certain permeability, so that culture conditions from the establishing of sterile explants to rooting and acclimatization stages are regulated and controlled, the photoautotrophical capacity of cultures is remarkably improved, and the quality and the seedling culture efficiency of tissue cultured seedlings are improved. Practices show that by virtue of the industrialized seedling culture method, the vitrification rate of explants can be decreased in an induction stage of the explants; in multiplication and expanding propagation stages, the proportion of vitreous buds can be reduced, and the quality of cultured seedlings is improved; in a rooting stage, the induced rooting time can be shortened, and the rooting rate is increased; and in acclimatization and transplant stages, the seedling domestication period is shortened, and the survival rate of transplanted seedlings is increased. Therefore, the industrialized production efficiency of the detoxified health sugarcane seedlings is finally integrally improved, and the production cost of cultured seedlings is lowered.