The invention relates to the technical field of pesticide, in particular to a weeding composition and preparation and application thereof.The composition has the advantages that effective active components cyhalofop-butyl and efficient and safe synergist of the composition have a good complementary effect within a given range, certain synergism is achieved, the preventive effect is obviously higher than that of a single preparation, and the using amount of pesticide is small.The weeding composition can have a special effect on gramineous weeds in paddy field.