The present invention discloses a desulfurization agent loading method, which belongs to the technical field of coke oven gas desulfurization. The method is as follows: multiple independent layers are repetitively loaded, each individual layer from bottom to top in turn includes a support network, a coke layer, an isolation network and a desulfurization agent layer, each individual layer is separately packed in a hanging frame with a height of 1m, the hanging frame, a tank bottom and an adjoining hanging frame are sealed by an asbestos material. According to the loading method, by cross-loading combination and matching of the coke layer and the desulfurization agent layer, coke oven gas entering a filler layer is fully contacted, and is freely permeable, the desulfurization effect of the coke oven gas is increased, gas flow variation caused by filler layer agglomeration can be reduced, and the cleaning effect of the coke oven gas is improved.