The invention discloses a bursting strength detection device of a sealant for a surgical operation. The bursting strength detection device comprises a matrix fixing cavity (1), a matrix fixing ring (2), a boosting pump (3), a pressure sensor (4) and a recorder (6), wherein the matrix fixing ring (2) is fixed on the matrix fixing cavity (1); an opening is formed in the upper end of the matrix fixing cavity (1) and the bottom of the matrix fixing cavity (1) is connected with the boosting pump (3) and the pressure sensor (4) through a three-way pipeline (5) respectively; and the pressure sensor (4) is connected with the recorder (6). The bursting strength detection device of the sealant for the surgical operation, disclosed by the invention is simple in structure and convenient to use, and has the characteristic of safety, reliability, environmental friendliness, convenience and the like.