The present invention discloses a 10kV line segment safety isolation method. The method comprises the following steps: (1) breaking an isolation switch at the receiving end of a transmission line to allow the isolation knives of the isolation switch to rotate downwards so as to break away from a movable contact; (2) employing the line segment isolation knife anti-maloperation power transmission locker to lock the isolation knives of the isolation switch, the line segment isolation knife anti-maloperation power transmission locker consisting of an insulated cross bar body, a hook being arranged at the upper end of the vertical bar of the cross bar body, a hoop with a lock being connected at the lower end of the vertical bar of the cross bar body, the hook of the locker being hooked on a cross arm to allow the cross bar of the cross bar body to be located at the upper portion of all the isolation knives, the hoop being locked on an electric power bar. The 10kV line segment safety isolation method is able to lock isolation knives after a ling segment isolation switch is disconnected and has good safety.