The invention relates to an indoor breeding method of fry of rhinogobio ventralis sauvage et dabry, and belongs to the technical field of breeding of rare and endemic fishes.The method comprises the steps that according to physiological and ecological characteristics of rhinogobio ventralis sauvage et dabry at different development stages of fry, advanced fry and juvenile fishes and differences of sensitivity to main pathogenic microorganisms of ichthyophthirius multifiliis, the whole fry breeding process is divided into three stages of tending barrel micro aeration breeding, tending barrel temperature-control microflow water breeding and glass fiber cylinder imitation-ecology mixed breeding.According to the fry breeding method of the rhinogobio ventralis sauvage et dabry, artificial regulation is conducted on critical water quality environmental factors of plankton communities and water temperature on the basis of intensively studying an influence mechanism of environmental factors causing ichthyophthirius multifiliis, a traditional pond water quality regulation technology is applied to a microflow water breeding mode, a series of technical problems that in the fry breeding process, stress responses of the fry are intense, ichthyophthirius multifiliis occurs frequently, and the fry grow slowly are effectively solved, and a technical support is provided for large-scale breeding of the fry of endemic fishes in the upper Yangtze River.