The invention relates to an agricultural insecticidal composition which takes bacillus thuringiensis and beauveria bassiana as main active components and an application thereof. The agricultural insecticidal composition contains active medical components, a pesticide carrier and an auxiliary agent, wherein the active medical components comprise the bacillus thuringiensis and the beauveria bassiana and the weight part ratio of the bacillus thuringiensis to the beauveria bassiana is 1 to (1-6). The agricultural insecticidal composition provided by the invention has high efficiency and low toxin, and is safe to human, livestock and crops; the crops can be protected and natural enemies are not injured, so that the influences on the ecological environment are small, and a development direction of current pesticides is met; and furthermore, the agricultural insecticidal composition is a green pesticide preparation which has an extremely good development potential.