The invention relates to the technical field of fruit and vegetable preservation and discloses a room temperature storage and preservation operation method for red kiwi fruits. The method comprises the steps of fresh fruit picking, preservative preparation, fresh fruit soaking and fresh fruit preservation. A preservative suitable for storage and preservation of red kiwi fruits is obtained by screening proper preservatives and can be popularized to be used for other fresh fruits, and the defects that a traditional preservative is poor in preservation effect, large in spraying dosage and large in toxic and side effect on the human bodies are overcome.