The invention discloses a high-accuracy automatic feeding mechanism applied in an LED screen plug-in mounting machine. The high-accuracy automatic feeding mechanism comprises a carrying mechanism and a clamping mechanism positioned on the surface of the carrying mechanism, the carrying mechanism comprises a first conveying device and a second conveying device which are arranged symmetrically, the clamping mechanism comprises a left fixing device and a right fixing device which are symmetrically arranged on the upper portions of the first conveying device and the second conveying device respectively, and LED lamp beads are clamped between the left fixing device and the right fixing device. The high-accuracy automatic feeding mechanism has the advantages that by using the above scheme, the high-accuracy automatic feeding mechanism is high feeding speed and low error rate, thereby being capable of meeting needs of large-scale production of LED screens.