The invention provides diazido-substituted poly(tetraethylene glycol), and the structural formula is as follow (please see the formula in the description).The synthesized diazido-substituted poly(tetraethylene glycol) can promote proliferation and migration of fibroblasts and epithelial cells, promote expression of collagen I protein in the cells, stimulate cell factors including NF-kappaB, STAT3, AP1 and Myc which are included in the cells and related to an inflammatory reaction to comprehensively play a role in wound healing promotion and enable the cell factors to achieve the significant treatment effect at the extremely low dosage.Therefore, the diazido-substituted poly(tetraethylene glycol) is likely to change the market pattern of existing wound healing promotion drugs and becomes a clinical drug which can be used for a long term, effectively promote wound healing and improve the healing effect.