The invention discloses a method for preparing a polyaniline nano-tube/graphene composite with glucose as a template. The method comprises the steps of dispersing aniline into an aqueous solution containing glucose, then adding self-made graphene oxide, after the materials are dispersed uniformly, adding ammonium persulfate for triggered polymerization, conducting a reaction for 12 hours at the room temperature, and adding glucose again for sufficiently reducing graphene oxide, so that the polyaniline /graphene composite of a nanometer tubular structure is obtained. According to the method, glucose plays two roles in the reaction process, on one hand, aniline is subjected to hydrogen bond self-assembly with glucose as the template, and subsequently, the composite of the tubular structure is formed by aniline and graphite oxide through oxidation polymerization; on the other hand, the green reducing agent, namely glucose, can reduce graphene oxide. The prepared polyaniline /graphene composite not only is of the nanometer tubular structure but also has good electrochemical performance. The method is simple in preparation process and environmentally friendly.