The invention discloses a humulus japonicus glutinous rice wine and a preparation method thereof. The wine is prepared from the following raw materials in parts by weight: 35-40 parts of humulus japonicus juice, 100-120 parts of glutinous rice, 5-7 parts of rice wine koji, 15-20 parts of rock candy, 0.05-0.08 part of citric acid, and an appropriate amount of water. The production process of the wine comprises the following steps: preparing the humulus japonicus juice, washing, soaking, steaming and cooling the glutinous rice, adding the humulus japonicus juice and saccharomyces cerevisiae, stirring the mixture, and performing primary fermentation, after fermentation, aging, clarification, filtration, and sterilization to obtain a finished product. The rice wine disclosed by the invention contains humulus japonicus, and compared with the conventional rice wine, the rice wine disclosed by the invention not only has a cool, refreshing and delicious mouthfeel, but also has double actions of nourishing and health protection.