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发明授权
CN105734130B 全基因组范围的植物基因功能鉴定的方法 有权

全基因组范围的植物基因功能鉴定的方法
摘要：
本发明涉及全基因组范围的植物基因功能鉴定的方法，包括：1)将出发植物进行单核苷酸突变，构建植物突变体库；2)检测步骤1)所述植物突变体库中突变体的表型，构建植物突变体表型数据库；3)检测步骤2)所述植物突变体库中突变体的基因型，构建植物突变体基因型数据库；4)结合植物突变体表型数据库和植物突变体基因型数据库，确定出发植物中由所述单核苷酸突变导致的SNP位点所在基因的功能。本发明建立的植物突变体表型数据库和突变体基因型数据库，可以精确、迅速地找到与植物发育过程、抗逆反应或代谢途径等生物学过程相关的基因，为植物功能基因组研究开创了新途径。
摘要（英）：

The invention relates to a method for identifying plant gene functions in total genome ranges.The method includes 1), mutating single nucleotide of starting plants and establishing plant mutant libraries; 2), detecting phenotypes of mutants in the plant mutant libraries and establishing plant mutant phenotype databases; 3), detecting genotypes of the mutants in the plant mutant libraries and establishing plant mutant genotype databases; 4), determining functions of genes where SNP (single nucleotide polymorphism) sites caused by mutation of the single nucleotide in the starting plants by the aid of the plant mutant phenotype databases and the plant mutant genotype databases.The method has the advantages that the genes related to plant development procedures and biological procedures of stress-tolerant reaction or metabolic pathways or the like can be accurately and quickly found by the aid of the plant mutant phenotype databases and the plant mutant genotype databases which are established by the method, and novel ways can be created for plant functional genome research.

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