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发明授权
CN105734148B 一种对普通小麦Glu-A1基因座位不同等位变异进行分型的方法和应用 有权

一种对普通小麦Glu-A1基因座位不同等位变异进行分型的方法和应用
摘要：
本发明公开了一种鉴别普通小麦Glu‑Al基因座位不同等位变异的方法和应用，本发明基于小麦Glu‑Al基因座位序列特征开发出2个序列标签位点(sequence‑tagged site，STS)标记Xid3和Xid4及相应的引物对(SEQ ID No.1和SEQ ID No.2；与SEQ ID No.3和SEQ ID No.4)，根据标记Xid3与Xid4的扩增多态性所形成的单元型(haplotype)差异可以有效区分普通小麦Glu‑Al基因座位Glu‑Ala，Glu‑Alb和Glu‑Alc三种等位形式。本发明简单、稳定、易行，是目前已知唯一可以同时对普通小麦Glu‑Al基因座位三种等位形式进行基因分型的分子标记。
摘要（英）：

The invention discloses a method for verifying common wheat Glu-A1 gene locus different allelic variation and application.Based on wheat Glu-A1 gene locus sequence characteristics, 2 sequence-tagged sites (STS) are developed to mark Xid3 and Xid4 and corresponding primary pairs, namely, SEQ ID No.1 and SEQ ID No.2, and SEQ ID No.3 and SEQ ID No.4, and the three allelic forms of common wheat Glu-A1 gene loci Glu-Ala, Glu-Alb and Glu-Alc can be effectively distinguished according to the variation of the haplotype formed by amplification polymorphism of the marked Xid3 and Xid4.The method is simple, stable and easy to implement, and is the unique known method through which genetic typing can be carried out on the three allelic forms of the common wheat Glu-A1 gene loci at the same time at present.

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