The invention discloses a molecular imprinting sensor and a detection method for bisphenol A. According to the method, on the surface of a sensitivity enhancing material nano gold modification electrode, an assembling method and a surface sol-gel method are combined to prepare the molecular imprinting sensor for testing a titanium dioxide molecularly imprinted membrane of bisphenol A. With the combination of the sensitivity enhancing function of nano gold and the surface molecule imprinting method, potassium ferricyanide is taken as an electrochemical probe, and thus bisphenol A can be rapidly, sensitively and specifically detected. Detection on other substrates can be achieved by only changing template molecules. The detection method is applicable to an electrochemical molecular imprinting sensor method to test bisphenol A.