The invention relates to an uninterruptible power supply rectification control interface board test system and method. The method comprises the steps that a front-end processor analyzes test case information into an excitation configuration instruction, a channel configuration instruction, a sequential control instruction and a signal acquisition instruction; a relay tester and a DC adjustable power supply respectively output corresponding AC and DC voltage excitation signals; a temperature and humidity measurement and control board acquires temperature and humidity information and acquires the DC voltage excitation signals and power supply signals; an acquisition control device accesses the power supply signals and the AC and/or DC voltage excitation signals to an acquisition adaptation device; the AC voltage excitation signals are acquired; the acquisition adaptation device performs conditioning on the AC and/or DC voltage excitation signals and inputs the AC and/or DC voltage excitation signals to a rectification control interface board; response signals outputted by the rectification control interface board are acquired; and the front-end processor processes and stores the acquired signals. With application of the technical scheme, work personnel of test personnel can be enhanced and maintenance cost can be reduced.