The invention provides a high-temperature proton exchange membrane fuel cell membrane electrode, which an acid-doped high-temperature proton exchange membrane and acid-doped gas diffusion electrodes, wherein the acid-doped gas diffusion electrodes are arranged at two sides of the acid-doped high-temperature proton exchange membrane; the mass percentage of the acid doping amount in the high-temperature proton exchange membrane is 150-200wt.%; the acid doping amount of the unit electrode area in the gas diffusion electrodes is 1-10mg/cm<2>; and the preparation method of the high-temperature proton exchange membrane fuel cell membrane electrode comprises the following steps: carrying out acid soaking treatment and aftertreatment of the membrane to constant weight; uniformly arranging the acid-doped solution on the surface of each gas diffusion electrode and removing a solvent through vacuum drying; and carrying out hot-pressing and cooling on the acid-treated gas diffusion electrodes and the acid-doped membrane and then obtaining the high-temperature proton exchange membrane fuel cell membrane electrode. According to the method, the problem of the uncertainty of the effective acid doping amount in the proton exchange membrane can be effectively solved; and the membrane electrode preparation consistency is ensured.