The invention discloses an indoor large-scale artificial breeding method for semiaquatic fire flies. The indoor large-scale artificial breeding method comprises the following steps: (1) constructing a breeding room; (2) configuring breeding facilities; (3) hatching eggs; (4) breeding larvae; (5) managing in the pupal period; (6) breeding at the adult stage and the oviposition stage. By adopting the indoor large-scale artificial breeding method, artificial indoor large-scale efficient three-dimensional circulation breeding of the semiaquatic fire flies can be achieved.