The invention discloses a traditional Chinese medicinal composition for tonifying qi, nourishing yin, invigorating pulse-beat and relieving pains, and a preparation method thereof, and belongs to the field of traditional Chinese medicine modernization. Active components of the traditional Chinese medicinal composition for tonifying qi, nourishing yin, invigorating pulse-beat and relieving pains are prepared from Radix Ophiopogonis, licorice root and Polygonum multiflorum. Compared with present commercial dosage forms, the composition disclosed in the invention has the advantages of stable and controllable quality, substantially improved curative effect, reduction of the taking dosage of patients, improved bioavailability, and substantial economic and social benefits.