The application discloses a microorganism culture plate. The microorganism culture plate has a top end for supplying a liquid in the vertical direction and a bottom end for discharging the liquid, and a first side and a second side which are connected to the top end and the bottom end and are arranged in the transverse direction perpendicular to the vertical direction. The microorganism culture plate also comprises a first film layer and a second film layer, and also comprises a first external surface and a second external surface which are opposite to the first film layer and the second film layer. A target microorganism to be cultured is fixed on the first external surface and the second external surface. The first film layer and the second film layer have micropores suitable for a liquid to penetrate. The first film layer and the second film layer are sealingly connected at the first side and the second side to form a hollow internal space. A liquid supplied from the top end to the internal space penetrates through the first film layer and the second film layer to reach the first external surface and the second external surface.