The invention discloses a bacterium inoculation tool and relates to the technical field of microbial culture.The bacterium inoculation tool is designed for solving the problem that use convenience is poor.The bacterium inoculation tool comprises an inoculating needle, an inoculating loop and a containing box containing the inoculating needle and the inoculating loop.The containing box is of a columnar box structure with two open ends.A head cavity for containing a needle portion of the inoculating needle and a loop portion of the inoculating loop and at least two tail cavities for containing a handle of the inoculating needle or a handle of the inoculating loop are formed inside a box body.The tail cavities are independent from each other.A front-end limiting piece and a tail-end limiting piece are fixedly arranged inside each tail cavity.A tail cover is installed at the end of each tail cavity.A guiding through hole is formed in each tail cover.A columnar button sliding along the axis of the guiding through hole is formed inside each guiding through hole in a matched mode.The connecting ends of the buttons are tightly connected with the handle of the inoculating needle or the handle of the inoculating loop and can be connected with the front-end limiting pieces and the tail-end limiting pieces in a matched mode.The free ends of the buttons are located outside the containing box.The bacterium inoculation tool can obviously improve the use convenience.