The invention discloses a human serum adiponectin detection kit based on micro-particle chemiluminescence immune assay, and relates to the chemiluminescence detection kits. The human serum adiponectin detection kit comprises reaction buffer liquor, magnetic micro-particles coated with an adiponectin monoclonal antibody, the adiponectin monoclonal antibody marked with acridinium ester, luminescence liquor A, luminescence liquor B, an adiponectin antigen standard substance solution and concentrated scrubbing solution. A preparation method for the human serum adiponectin detection kit comprises the following steps: preparing the reaction buffer liquor; preparing the magnetic micro-particles coated with the adiponectin monoclonal antibody; preparing the adiponectin monoclonal antibody marked with acridinium ester; preparing the luminescence liquor; and preparing the scrubbing solution. According to the human serum adiponectin detection kit, the magnetic micro particles are coupled with the monoclonal antibody, and the micro particles can be suspended in a sample solution, so that the antibodies on the surfaces of the micro particles are sufficiently in contact with a to-be-detected object, and the reaction time is shortened; and the acridinium ester is used as the marker, so that a stable and high-strength signal can be obtained. The human serum adiponectin detection kit is simple to operate, is high in sensitivity, is wide in linearity range, is stable in result, is good in safety and is convenient for automation.