The invention discloses a toothpaste composition with an antibacterial function. The toothpaste composition comprises a grinding agent, a humectant, an adhesive, a diester type surfactant, compound enzymes, a sweetener, perfume, and deionized water. Biological enzymes decompose the extracellular polymers excreted by floras of dental plaque, cell walls and cell membranes, and at the same time generates hydrogen peroxide and hydroperoxide during the decomposition process; hydrogen peroxide and hydroperoxide can oxidize and neutralize high level mercapto groups in the biological membranes of dental plaque, oxidize and kill cells of bacteria at the same time, and reduce the resistance of bacterium cells to exogenous biological enzymes; at the same time, the diester type surfactant has a strong cleaning and stain-removing ability, and is capable of strongly removing biological membranes of dental plague and bacterium residues; after the using of the toothpaste, the average colony-forming units are reduced by 79.1 to 83.2%, and the number of bacteria is obviously reduced.