The invention discloses a nanometer 1H-19F-31P multi-nuclear magnetic resonance molecular imaging probe transmitted through the respiratory tract and a preparing method thereof and belongs to the technical field of medical diagnosis.The preparing method comprises the steps of 1, evenly mixing one or more phospholipids surface active agents, dissolving the mixture with chloroform or a chloroform and methyl alcohol mixed solvent, and conducting drying and dispersion in water in sequence to obtain a phospholipids surface active agent blend; 2, evenly dispersing perfluoro-carbon and glycerinum in the surface active agent blend; 3, removing components not effectively coated, and conducting purification to obtain the probe.Researches show that after the probe is transmitted through the respiratory tract, tumor lesion position multi-nuclear imaging information can be provided, the dosage of an imaging contrast agent is reduced, and lung cancer molecular imaging in vitro detection effect is improved.