The invention relates to polysaccharide styptic powder, a preparation method and applications thereof. Plant origin starch and etherified starch are taken as the raw materials, two starches are individually processed by ultrasonic waves, then two starches are emulsified and crosslinked, and finally the crosslinking product is subjected to extraction, liquid separation, washing, drying, and sterilization to obtain the styptic powder. The styptic powder has a good biocompatibility, can be directly applied to the common bleeding parts, deep bleeding parts, and bleeding parts that are hard to reach through surgery operation, and is simple and convenient to use. Starches are the raw materials of the styptic powder, thus the styptic powder is nontoxic and nonirritant, moreover, the sources of raw materials are wide, the cost is low, the technology is simple and feasible, and thus the styptic powder has a wide clinical application prospect.