The invention discloses an automatic assembly machine of a production guide tube type tampon. The automatic assembly machine comprises a base, an assembly table, a controller, a first manipulator, a second manipulator, a third manipulator, a rotary manipulator, a first charging barrel, a second charging barrel and a third charging barrel, wherein a bracket is arranged on one side of the base, the controller is installed on the bracket, the rotatable assembly table is installed in the center of the upper surface of the base, the first manipulator, the second manipulator and the third manipulator are distributed around the assembly table, the first manipulator, the second manipulator and the third manipulator are respectively fed by the first charging barrel, the second charging barrel and the third charging barrel, and the rotary manipulator which is used for moving out an assembled tube is arranged on one side of the base. A small tube, a middle tube and a large tube of the production tampon can be assembled into a whole in sequence, the automatic assembly is realized, and the assembly efficiency is improved.