The invention relates to an environment-friendly preservation box and a preservation method for instant fresh-cut fruits and vegetables, and belongs to the technical field of preservation of the fruits and vegetables. The environment-friendly preservation box for the instant fresh-cut fruits and vegetables comprises a box body and a cover body (1), the box body comprises a box body framework (3) and a double-layer surrounding body (5) covering the outer side of the box body framework, and silica gel windows (4) are arranged on the side wall of the box body; the cover body is provided with a top opening (2), and a gasket is arranged on the top opening and contains ethylene absorbing agents; the double-layer surrounding body (5) comprises an inner-layer surrounding body and an outer-layer surrounding body, wherein the inner-layer surrounding body is made of degradable plastic with high moisture permeability, the outer-layer surrounding body is made of PE preservation films, and the part between the inner-layer surrounding body and the outer-layer surrounding body is filled with foam. The environment-friendly preservation box for the instant fresh-cut fruits and vegetables is high in air permeability and moisture permeability, the deterioration speed of the fruits and vegetables is low, and freshness of the fruits and vegetables is guaranteed.