Disclosed is a manufacturing method of a shape-controllable flexible micro-nano column array.The manufacturing method includes: coating a hydrosol adhesion layer on the surface of a substrate, obtaining a micro-nano graphical photoresist on the hydrogel adhesion layer through a photoetching process, obtaining micro-nano graphical particles on the surface of the photoresist through a scraping-coating method, and removing the photoresist to obtain a graphical template of the micro-nano particles; coating a polymer on the surface of the substrate, and pre-heating the substrate; enabling the micro-nano particles on the surface of the graphical template to contact with and to be embedded in the polymer; lifting the graphical template to enable the polymer to be drawn to be a micro-nano column array, and heating the substrate to solidify the micro-nano column array; heating the graphical template to enable the micro-nano particles to break away from the hydrogel adhesion layer to obtain a micro-nano column array with the micro-nano particles at the top end; removing the micro-nano particles at the top end of the micro-nano column array to obtain a polymer micro-nano column array.The manufacturing method has the advantages of simple operation, low cost and short period.