The invention belongs to the technical field of graphene, and in particular, relates to a stripping agent for liquid phase stripping preparation of graphene and an application thereof. The stripping agent is composed of the following components by the weight percentage: 75-80% of polymer nano microspheres, 10-15% of a chain extender, 5-10% of a dispersing agent and 1-5% of a catalyst. The components are mixed evenly in a high-speed stirrer, and the chain extender, the dispersing agent and the catalyst are evenly dispersed in the polymer nano microspheres; in the process of liquid phase stripping preparation of graphene, the stripping agent and expanded graphite are mixed to form a dispersion liquid; in a microwave heating process, a polymer interspersed between the graphite layers is subjected to a chain extending reaction, gaps between the graphite layers are expanded, the acting force between the graphite layers is reduced, at the same time, the dispersing agent adsorbed in the polymer microspheres can effectively prevent reunion of the graphene, and the dispersion of the graphene is improved. The graphene prepared by the stripping agent has high yield, the number of layers is less than or equal to ten, the structure defects are small, and the graphene has the prospect of market application.