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CN105777700A 一种分离生育酚同系化合物的方法 有权

一种分离生育酚同系化合物的方法
摘要：
本发明提供了一种生育酚同系化合物的分离方法，所述方法包括如下步骤：(1)将一种或多种极性溶剂与一种或多种非极性溶剂充分混合后静置分相，获得上相溶剂和下相溶剂；(2)将生育酚混合物溶解于所述步骤(1)获得的上相溶剂，作为待分离样品；(3)以所述步骤(1)获得的上相溶剂作为固定相，以所述步骤(1)获得的下相溶剂作为流动相，通过高速逆流色谱对步骤(2)的待分离样品进行分离，得到生育酚各同系化合物与流动相的混合液；其中，所述生育酚各同系化合物分别为α-生育酚，(β+γ)-生育酚和δ-生育酚；(4)去除所述步骤(3)获得的生育酚各同系化合物与流动相的混合液中的流动相溶剂，获得生育酚各同系化合物产品。
摘要（英）：

The invention provides a separation method of tocopherol homologous compounds. The method comprises the steps of: (1) mixing well one or more polar solvents with one or more non-polar solvents, and standing for phase separation to obtain an upper phase solvent and a lower phase solvent; (2) dissolving the tocopherol mixture in the upper phase solvent obtained in the step (1) as a sample to be separated; (3) using the upper phase solvent obtained in the step (1) as a stationary phase and the lower phase solvent obtained in the step (1) as a mobile phase, separating the sample to be separated in the step (2) by a high-speed countercurrent chromatography to obtain a mixed solution of tocopherol homologous compounds and the mobile phase, wherein the tocopherol homologous compounds comprise alpha-tocopherol, (beta + gamma)-tocopherol and delta-tocopherol; and (4) removing the mobile phase solvent in the mixed solution of tocopherol homologous compounds and the mobile phase obtained in the step (3), so as to obtain tocopherol homologous products.

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