The invention relates to a method for preparing environment-friendly anti-fingerprint resin. The method comprises the steps: (1) preparing a micromolecular chain extender by an esterification reaction and a nucleophilic addition reaction; (2) subjecting the micromolecular chain extender to a reaction with isocyanate, so as to prepare a polyurethane prepolymer with the terminal group of -NCO; (3) terminating the polyurethane prepolymer by using pentaerythritol triacrylate, thereby obtaining the environment-friendly anti-fingerprint resin. The environment-friendly anti-fingerprint resin prepared by the method can be used for directly coating the surface of a touch screen display panel, the surface of glass paper, the surface of an acrylic plate, the surface of polysulfone and the surface of tinplate without additionally adding any organic solvent and organic diluent, so that the environment-friendly anti-fingerprint resin is green and environment-friendly. The environment-friendly anti-fingerprint resin prepared by the method can be cured by an electron beam, and a coating layer, formed after the environment-friendly anti-fingerprint resin is cured, has good anti-fingerprint performance, so that the environment-friendly anti-fingerprint resin can be applied to the anti-fingerprint treatment of surfaces of materials such as the touch screen display panel, the glass paper, the acrylic plate, the polysulfone and the tinplate.