The invention discloses a potato culture medium suitable for ustilaginoidea virens spore production and an application thereof. Potatoes are prepared into a plate culture medium as a spore production culture medium so as to culture and prepare ustilaginoidea virens thin-wall conidiospore. Preparation for the culture medium and an application method for the culture medium comprise the following steps: 1) preparing the culture medium; 2) inverting a culture flat plate; 3) implanting ustilaginoidea virens; 4) carrying out constant-temperature culture; and 5) collecting a new generation of spores. The potato culture medium has the characteristics and the advantages that: the culture medium is simple in composition; the operation is simple, easy and convenient, and shaking culture equipment is not needed; time of obtaining a great number of spores is short; a high spore production state lasts for a long time; and a spore liquid is relatively pure, is less in mixed mycelia, and is generally suitable for being used in a lot of research work without purifying treatment.