The invention provides a waste tire rubber powder asphalt separation test and an evaluation method. The method comprises the following steps: firstly, drawing a waste tire rubber powder asphalt standard density graph; secondly, measuring the density of separated waste tire rubber powder asphalt, wherein the operation is needed only in case of separation of the waste tire rubber powder asphalt; thirdly, calculating corresponding content of waste tire rubber powder; further calculating the content difference of the waste tire rubber powder; finally, judging the separated waste tire rubber powder asphalt. The method aims to detect the separation degree of the waste tire rubber powder asphalt and judge whether the separated waste tire rubber powder asphalt can be directly used or not. A main experimental instrument used in the method is a common pycnometer, no special experimental instrument is needed, tests and calculation methods are simple, the evaluation method is easy to master and is practical and rapid, construction can be accurately instructed, waste can be avoided, and the efficiency can be improved.